Polyclonal Antibody Production in Mice, Rats and Guinea pigs

The animals are housed in our own animal department in Germany (Europe). This ensures animal friendly and cost efficient antibody production. Frequently individual observing of each animal and the titer development ensures high qulity antibodies with best titers. BALB/c mice and black hooded rats are used for the antibody production. To complete our services for small rodents the immunization of guinea pigs is available. The advantage of rats and guinea pigs are the larger amount of antiserum from one animal.
For monoclonal projects please go to our Monoclonal Development Page.

Antigen Preparation

Antigen Requirements

Immunization

Immunization Schedules

Antibody Purification

Available Purifications

Additionals Services

Additional Services

Order

Order Process


Antigen Requirements

For optimal immunization success the immunization of multiple mice, rats or guinea pigs with different antigen concentrations is recommended to receive best results. The average antigen amount needed for the immunization of one animal is listed in the table below. Depending which kind of antigen you choose, the amount of antigen might be different. Davids already successfully generated antibodies with even less amount of the recommended antigen . If you have less antigen please contact us: info@davids-bio.com. For further information click on the desired antigen.

Antigens for Small Rodent Antibody Production
Antigen 1 Mouse 1 Rat 1 Guinea pig Description
ProteinProtein20 - 250 µg
0.3 - 2 mg/ml
20 - 250 µg
0.3 - 2 mg/ml
0.15 - 0.5 mg
0.3 - 2 mg/ml
Customer Provided Proteins
Expression in E. coliProtein Expression---Protein Expression from your plasmid
SDS-gelProtein (SDS-Gel)25 - 250 µg
3 - 8 gel bands
0.15 - 0.3 mg
3 - 8 gel bands
0.15 - 0.6 mg
3 - 8 gel bands
Protein Extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel.
PeptidePeptides1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
Customer provided peptides or Peptide synthesis and Conjugation Service by Davids.
CellCells5 x 10 cfu5 x 107 cfu5 x 107 cfuPreparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system.
DNADNA1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
1 - 2 mg
0.3 - 2 mg/ml
DNA preparation for the immunizations by Davids
Conjugation of a small moleculeSmall Molecules1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
1 - 5 mg
0.3 - 2 mg/ml
Preparation of small molecules by Davids
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

Immunization Schedules for Small Rodents

Our well established 63-day immunization schedule offers the best results in titer development and affinity for most antigens. For your special needs we are able to follow your individual immunization protocols or immunization techniques. Longer immunization times, additional immunizations, additional test bleeds or special immunization techniques are available. For large projects with a high number of mice, rats or guinea pigs please ask for your special offer: info@davids-bio.com.


Immunization Protocols
Mouse Rat Guinea Pig
Cat.NoA401 (1 mouse)
A402 (2 mice)
A404 (4 mice)
A205 (1 rat)
A206 (2 rats)
A201 (1 guinea pig)
A202 (2 guinea pigs)
MouseRatGuineapig
Schedule63-day63-day63-day
Schedule ProlongationPossiblePossiblePossible
Immunizations555
Titer Determinationday 35day 35day 35
Serum Amount0.2 - 1 ml2 - 10 ml5 - 15 ml
Purification Options
PurificationPrecipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Precipitation (R001)
Affinity Purification (R007)
ProteinA (R008)
Antigen Requirements
Suitable AntigensProtein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Protein
Protein (SDS-Gel)
Peptide
DNA
Cells
Small Molecules
Antigen Needed20 - 250 µg Protein20 - 250 µg Protein0.15 - 0.5 mg Protein
Antigen BufferNon toxic bufferNon toxic bufferNon toxic buffer
PricePrice ListPrice ListPrice List
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Rodent Immunization Schedules
Day 63 Day Antisera
1Preimmune serum
11. Immunization
142. Immunization
283. Immunization
35
  • Test serum
  • ELISA-Titer determination
  • Optional: Test serum for the customer
424. Immunization
565. Immunization
63Anti serum
Get your offer to extend animal housing and the number of immunizations.

Antisera Purification

We offer different purification services for your polyclonal antisera from mouse, rat and guinea pig. We recommend to pool antisera from multiple mice before the purification to enhance the yield. Separate purifications from single antisera are available as well.
You have the choice of an affinity purification for specific antibodies and ProteinA purifications to receive a pure IgG fraction or depletions to get rid of unwanted antibodies that i. e. recognize tags.

Purification Options for Polyclonal Antibodies from Mice, Rats and Guinea Pigs
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (1 antiserum pool)
R007-2 (2 antisera)
R008 (1 antiserum pool)R003 (1 antiserum pool)R101 (anti-tag)
R102 (anti-E. coli)
Scheme of Antigen Specific Affinity PurificationScheme of ProteinA PurificationScheme of Affinity PurificationScheme of Antibody Depletion
Legend of Antibody Purification
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
Immuno-Histo-Chemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
Catalog PricePrice ListPrice ListPrice ListPrice List
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Additional Services

We offer multiple additional services for your special needs. Additional Immunizations, Test bleeds or animal housings are available. In addition we offer the preparation of cells from spleen, blood, bone marrow or lymph nodes for the production of monoclonal antibodies or RNA isolation.

Additional Services for Small Rodent Antibodies
Cat.No. Description Amount Catalog Price
A057Additional Small Bleed-Price List
A059Additional Immunization1 ImmunizationPrice List
A705ELISA Raw Data incl. graphFrom 1 BleedPrice List
A706Animal Housing1 day, 1 animalPrice List
N101Spleen Cell Isolation1 spleenPrice List
N201PBMC Lymphocyte Isolation1 antiserumPrice List
N301Bone Marrow Cell Isolation1 animalPrice List
N401Lymph Node Cell Isolation1 animalPrice List

Order Process

Davids keeps the order process very simple. Just send your antigen to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day the antigen arrives. Please provide the following information to fasten up the start and to help us connect your antigen with the correct order:

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies