Antigen Conjugation for Antibody Production
Different conjugation strategies are proposed to improve the antigenicity and epitope presentation of small molecules. When an antigen is attached to a carrier such as KLH, Ovalbumin, Thyroglobulin, BSA, or Dextran, its antigenicity increases. As a small molecule, DNA is treated differently. The preparation of the DNA for vaccination takes many processes. For further information, please send us an email.
Small Proteins
A conjugation of the antigen to a carrier is suggested for a stronger immune response with small proteins. First and foremost, this guarantees that the antigen appears larger. Furthermore, multiple epitopes are presented on a single carrier. Conjugated small proteins of < 15 kDa often elicit a considerably stronger immune response.
 Conjugation |
 Mixed Conjugation |
|
|---|---|---|
| Cat.No | P105 (1 protein) | P111 (1 protein) |
| Antigen | Protein from Customer | Protein from Customer |
| Recommended for | Small Proteins < 15 kDa | Small Proteins with low Antigenicity |
| Conjugation | 1 Conjugation | 3 Conjugations |
| Carrier | KLH BSA Ovalbumin Thyroglobulin Dextran More | KLH BSA Ovalbumin Thyroglobulin Dextran More |
| Conjugation with | Hydroxylation (-OH) Sulforylation (-SH) Carbonylation (C=O) Carboxylation (-COOH) | Hydroxylation (-OH) Sulforylation (-SH) Carbonylation (C=O) Carboxylation (-COOH) |
| Price | Price List | Price on demand |
 Get your free offer now | ||
DNA as Antigen
Davids prepares your DNA for direct immunization. Please contact us for more information: info@davids-bio.com.
Small Molecules & Toxins
To improve antigenicity, small molecule conjugation with carriers is highly recommended. Please provide us with the antigen structure so that we can determine the best carrier and conjugation strategy for the antigen.
Toxins should be introduced into liposomes to minimize toxicity. Take advantage of our toxin immunization protocol.
Order Process
You can get your quotation for the preparation of small molecules here: info@davids-bio.com. We recommend to contact us before you send small molecules to receive the best options available for your antigen. Immunizations usually begin on the same day as the antigen arrives. Please submit the following information to assist us correlate your antigen with your order and accelerate the process
- If available: Name and Structure of your antigen
- If available: DNA sequence, DNA length
- Known Information of your antigen: Solubility, Toxicity (LD50)
- Concentration of your antigen and buffer
- Our Offer or your official PO
- VAT number (only EU Customers, not German Customers)
As a second option to small proteins you can use peptides that represent your protein. If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies
