Antigen Conjugation for Antibody Production

To enhance the antigenicity and the epitope presentation of small molecules the usage of different conjugation methods is recommended. The antigenicity can enhance, when the antigen is bound to a carrier like KLH, Ovalbumin, Thyroglobulin, BSA or Dextran. DNA as a small molecule is handled differently. The preparation of the DNA for the immunization is done in multiple steps. Please write us an email for further information.

Small
Proteins

DNA
 

Small Molecules
Toxins etc.

Order Process

Small Proteins

To receive a better immune response with small proteins a conjugation of the antigen to a carrier is recommended. First this ensures that the antigen is larger. In addition multiple epitopes are presented on one carrier. The immune response is usually much higher with conjugated small proteins < 10 kDa.

	Conjugation	Mixed Conjugation
Cat.No	P105 (1 protein)	P111 (1 protein)
Antigen	Protein from Customer	Protein from Customer
Recommended for	Small Proteins < 10 kDa	Small Proteins with low Antigenicity
Conjugation	1 Conjugation	3 Conjugations
Carrier	KLH BSA Ovalbumin Thyroglobulin Dextran More	KLH BSA Ovalbumin Thyroglobulin Dextran More
Conjugation with	Hydroxylation (-OH) Sulforylation (-SH) Carbonylation (C=O) Carboxylation (-COOH)	Hydroxylation (-OH) Sulforylation (-SH) Carbonylation (C=O) Carboxylation (-COOH)
Price	Price List	Price on demand
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DNA as Antigen

Davids prepares your DNA for direct immunization. Please contact us for more information: info@davids-bio.com.

Small Molecules & Toxins

A conjugation of small molecules with carriers is highly recommended to increase the antigenicity. Please provide us with the antigen structure so we can have a look what kind of carrier and conjugation method fits for the antigen.
For toxins we recommend the introduction into liposomes to reduce toxicity. Profit from our toxin immunization schedule.

Order Process

You can get your offer for the preparation of small molecules here: info@davids-bio.com. We recommend to contact us before you send small molecules to receive the best options available for your antigen.

To fasten up the process and help us to give you the best service possible ensure you provide the following information:

1. If available: Name and Structure of your antigen
2. If available: DNA sequence, DNA length
3. Known Information of your antigen: Solubility, Toxicity (LD₅₀)
4. Concentration of your antigen and buffer
5. Our Offer or your official PO
6. VAT number (only EU Customers, not German Customers)

As an alternative to small proteins you can use peptides that represent your protein. If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies