Peptide Synthesis & Peptide Antibodies

The major advantage of Peptide Antibodies is, that you can produce antibodies from proteins you only know the sequence from. There is no need for protein production or purification.

Peptide Predictions

Our scientists work with you to identify peptides that represent your protein in accordance with Davids peptide prediction algorithms (see info box). Use this free service by sending us an email with your one-letter amino acid sequence (txt, pdf, fasta or directly in the email). All of your information is kept private. We will only begin synthesis of your peptide after you have confirmed one or two of the proposed peptides for antibody generation.
In collaboration with our experts, our prediction algorithm suggests peptides that represent your protein and are ideal for antibody generation. These peptides must be antigenic, soluble, and have high epitope surface area. You can immunize one animal with two distinct peptides to increase your chances of using the peptide that best represents your protein. The likelihood of using the optimal peptide rises from 70% (1 peptide) to 90% (2 peptides) to 97%. (3 peptides).

Peptide Synthesis

Solid phase synthesis is used to produce peptides. Each peptide passes quality control using HPLC and MS. For immunizations, the purity ranges from 70 to 98 percent. If you want a higher purity, please request this service. The services described in the table below are designed specifically for antibody generation. Davids "All-Inclusive-Service" includes everything you need, including peptide synthesis and conjugation. Please request a different amount and purity of a peptide or just a synthesis, if you don't need antibodies.

	Stand Alone Synthesis	All Inclusive	Mixed Conjugation	High Purity
Cat.No	P104 (1 peptide)	P101 (1 peptide) P102 (2 peptides)	P111 (1 peptide)	P10X (1 peptide)
Schedule	4 - 5 weeks	4 - 5 weeks	4 - 5 weeks	4 - 5 weeks
Synthesis	up to 20 aa included	up to 20 aa included	up to 20 aa included	each aa
Maximal Length	up to 100 aa	up to 100 aa	up to 100 aa	up to 100 aa
Conjugation	No conjugation	1 Conjugation	3 Conjugations	No Conjugation
Carrier	-	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More	-
Peptide
Purity	> 70%	> 70%	> 70%	> 95%
Amount	15 mg	15 mg	15 mg	15 mg
Quality Control
HPLC	Included	Included	Included	Included
MS	Included	Included	Included	Included
Application
Recommended for	For your applications Affinity Purification Depletion	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Peptides with small antigenicity	Applications that require high purity
Price	Price List	Price List	Price List	On Demand
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Peptide Conjugation

Peptides are covalently conjugated to a carrier such as Key Limphole Hemocyanin (KLH), Ovalbumin, Thyroglobulin, Bovine Serum Albumin (BSA), or other molecules to increase antigen presentation from small molecules. Following immunizations, affinity purification is recommended in order to get monospecific antibodies. The conjugation is performed at the -NH₂, -COOH, -SH, -OH or other groups of the amino acids, depending which amino acids are available at the peptide. To enhance the antigenicity multiple carriers or multiple conjugation methods can be used. This Mixed Conjugation (P111) includes the peptide synthesis and is recommended for peptides with low antigenicity. Davids All-Inclusive-Services P101 and P102 include the peptide synthesis, conjugation and quality control as well.

	Stand Alone Conjugation	All Inclusive	Mixed Conjugation
Cat.No	P105 (1 peptide)	P101 (1 peptide) P102 (2 peptides)	P111 (1 peptide)
Schedule	1 day	4 - 5 weeks	4 - 5 weeks
Synthesis	Peptide from Customer	up to 20 aa included	up to 20 aa included
Maximal Length	-	up to 100 aa	up to 100 aa
Conjugation	1 Conjugation	1 Conjugation	3 Conjugations
Carrier	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More	KLH BSA Ovalbumin Thyroglobulin More
Peptide
Purity	-	> 70%	> 70%
Amount	-	15 mg	15 mg
Quality Control
HPLC	-	Included	Included
MS	-	Included	Included
Application
Recommended for	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Rabbit Antisera Mice, Rats, Guinea pig Antisera Chicken Egg Yolk Antibodies	Peptides with small antigenicity
Price	Price List	Price List	Price List
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Peptide Immunization

Davids begins vaccinations as soon as the peptide synthesis and preparation is completed. Please include one of our custom polyclonal antibody services for rabbits, chicken, mice, rats, guinea pigs, or one of our custom monoclonal antibody services for Mice or Rats in your purchase.
To get monospecific antibodies following peptide vaccination, we recommend affinity purification for polyclonal antibodies.

Additional Services

When using peptides for in vivo cell culture applications, additional services such as peptide preparation are recommended. The peptide fraction is diluted here, and impurities are removed using HPLC. Lyophilization is used to eliminate vaporous compounds. In a third stage, the peptide fraction is sterile filtered and diluted in a cell culture suitable buffer such as PBS or water.

Cat.No.	Description	Amount	Catalog Price
P201	Preparation of peptide for Cell Culture Use	1 Peptide	Price List

Order Process

Davids maintains the ordering procedure as simple as possible. Send us your amino acid sequence (one letter amino acid code as txt, pdf, or directly email) for peptide antibodies, and our scientists will predict antigenicity, solubility, and epitopes. Davids begins synthesis only once you confirm the peptide(s) through email, PO. If you wish to provide us peptides that you have produced in your lab, please email them to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day, when the antigen arrives. To fasten up the immunization and/or synthesis process and help us to connect your antigen/sequence with your order, ensure you provide the following information:

1. Your Peptide/Protein Sequence
2. Concentration & Buffer Conditions of your Peptide
3. Our Offer, our order form or your official PO
4. VAT number (only EU Customers, not German Customers)