Recombinant expression of your protein in E. coli is used to receive enough protein for the production of antibodies. As an alternative to protein expression in E. coli you can use peptides that represent your protein. Just send us your protein sequence and we suggest you peptides for the antibody production. For more information go to our Peptide Page.
Synthesis of your DNA and codon optimization for the expression in E. coli. Restriction sites are inserted to subclone the synthesized DNA in a vector. The DNA is subcloned in an expression vector. Here we usually use an IPTG induceable plasmid with ampicillin or kanamycin resistance and a tag for easy purification like HIS-tags.
To express your protein in the best possible way Davids performs an expression evaluation. The plasmid is transformed in different E. coli strains (two strains are included). The inductor concentration, the expression time and the expression temperature is varied.
This is only needed, when no expression protocol is available. When you provide us with an the expression plasmid and an expression protocol this step is not needed.
Davids performs the protein expression of your protein in E. coli after the protocol established in the Expression Evaluation or after a customer provided protocol. The expression is for the antibody production only. We can not guarantee that the final protein/enzyme stays active during the purification process.
You can get your offer for protein expression here: firstname.lastname@example.org. Every expression project is different and to give you the best service I recommend to write us your special needs.
To fasten up the process and help us to give you the best service possible ensure you provide the following information:
As an alternative to protein expression you can use peptides that represent your protein. If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies