Whole Cells as Antigen

Cells like bacteria, phages, fungi, other eukaryotic cells and even virus particles profit from a good presentation to the immune system. Especially when you want to detect the whole cell it is important, that the antibody recognizes the surface of the cell. With Davids Cell Preparation KIT the surface of the cells is presented in the best way to the immune system. Cells as antigen can be used for the whole immunization process or as a last boost to show the immune system the native extracellular antigens. This will lead to more specific antibodies in many cases.

Preparation of Cells for Antibody Production

For the optimal presentation of the antigen from the cells it is recommended to use Davids Cell Preparation KIT. You can do the cell preparation in your lab or send us your cells and let us perform the cell preparation prior to immunizations.

Whole Cell

Cell Preparation for Immunization

Purification

Antibody Purification

Additional Information

Additional Information

Order Process

Order Process


Preparation of whole cells for immunization

For optimal presentation of the cell antigens use our Cell Immunization KIT. You can do the preparation in your lab or send us your inactivated cells and we perform it in our lab. The price is the same. We highly recommend that you perform the preparation in your lab with fresh cells. Though when you want us to do it please inactivate your cells i.e. with heat or UV-light. For additional information please send us an email: info@davids-bio.com. Table 1 shows the cell amount needed for the immunization of one animal.

Recommended cfu/pfu for the immunization of one animal. The optimal amount of cells needed is different for each cell type.
Rabbit Chicken Mouse Rat Guinea Pig
Rabbit Antigen RecommendationsChicken Antigen RecommendationsMouse Antigen RecommendationsRat Antigen RecommendationsGuinea Pig Antigen Recommendations
Bacterial Cells (1 animal)5 x 107 - 5 x 108 cfu5 x 107 - 5 x 108 cfu5 x 107 cfu5 x 107 cfu5 x 107 cfu
Virus Particles (1 animal)107 pfu107 pfu107 pfu107 pfu107 pfu
Mammalian Cells (1 animal)107 - 108 cfu107 - 108 cfu107 - 108 cfu107 - 108 cfu107 - 108 cfu
Affinity ColumnAdditional 108 cfu needed to create an affinity column for antigen specific affinity purification
Cell PreparationPrice List

Purification of Antibodies

Antibodies from whole cell antigens can be purified with ProteinA or with affinity purification. For the affinity purification we need additional 108 cfu/pfu to create the affinity matrix. For an overview of our purification methods go to our Purification Page.

Additional Information

Please keep in mind that ELISA titer determinations with whole cells may not show the real titer. For an optimal titer determination the cells have to bind to the ELISA plate. Davids developed methods to bind cells to the ELISA plates, though different conditions are optimal for each species or cell type. The real titer from whole cell antigens is usually higher than determined with our ELISA. You can send us the protein antigen for ELSIA determinations, if you need a more precise titer and when the protein is available.

Order Process

Davids keeps the order process very simple. Please use our Cell Immunization KIT or inactivate your cells i.e. with heat or UV-light before you send them to us. Please contact us before you send infectious or pathological cells. You can send your inactivated whole cells to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The Cell Immunization Preparation with our Cell Immunization KIT is done in our lab when we receive cells that are not prepared for immunization. The immunizations usually start within 2 - 3 days after your cells arrive in our lab. To fasten up the immunization process and to help us connecting your antigen with the correct order ensure you provide the following information:

  1. Your Antigen
  2. Cell Amount
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies