Custom Antibody Production from Chicken Egg Yolk

The yield of chicken antibodies is very high. All antibodies are isolated from eggs. A huge amount of antibodies can be obtained over time as the amount of antibodies from 10 eggs is comparable with the amount of antibodies from one rabbit. This saves antigen and is very animal friendly as one chicken is able to produce 42000 mg antibodies in one year. Thus chicken antibodies are a very good alternative to sheeps and goats, or when you need a large amount of antibodies.
The antibody production from one chicken over one year can replace up to 20 rabbits, 4 goats or 4 sheeps. All egg yolk antibodies ordered from Davids are ready to use. Davids PrepI preparation from the egg yolk is comparable to raw sera from mammals. Additional purifications are available for your special needs. You find all advantages of Chicken Egg Yolk Antibodies here.

If you want to learn more about chicken egg yolk antibodies or when you are interested in a special offer for a large amount of antibodies just write us an email: info@davids-bio.com.

Antigen Preparation

Antigen Requirements

Immunization

Immunization Schedules

Antibody Purification

Available Purifications

Additional Services

Additional Services

Order

Order Process


Antigen Requirements

The antigen amount needed for chicken antibodies is very low compared to the amount of antibodies you can achieve. The table below shows our recommendations of antigens for the production of chicken egg yolk antibodies. Though, the antibody production can usually be performed with less antigen. In this case please ask us for this service. If you need further information or if you have another antigen that does not fit in the table please contact us.

Antigens for Chicken Antibody Production
Antigen Amount
1 chicken
Instructions Shipping
ProteinProtein0.5 mg
0.3 - 2 mg/ml
Customer provided proteins-20°C (ice packs) to -80°C (dry-ice) or lyophilized
Expression in E. coliProtein Expression-Expression Service by DavidsShip Expression Plasmid at -20°C
SDS-gelProtein (SDS-Gel)0.5 mg
3 - 8 gel bands
Protein Extraction from SDS-gel pieces. Cut the protein specific gel bands from the gel.Without addition of solutions at room temperature
PeptidePeptides1 - 2 mg
0.3 - 2 mg/ml
Customer provided peptides or Peptide synthesis and Conjugation Service by Davids.Lyophilized or with ice packs
CellCells106 - 107 cfu / immunizationPreparation with Davids Cell Immunization KIT for optimal presentation of the surface to the immune system.Please contact us when you want to send cells
DNADNA1 - 2 mg
0.3 - 2 mg/ml
DNA preparation by Davids.At room temperature or with ice packs
Conjugation of Small MoleculesSmall Molecules1 - 10 mg
0.3 - 2 mg/ml
Preparation of small molecules by DavidsPlease contact us when you want to send small molecules
For Affinity Purification we need additional >0.5 mg protein, 3 mg peptide or 108 cfu

Immunizations

The immunizations start as soon as your antigen arrives. Four immunizations are performed before the collection of the eggs and the preparation of the antibodies start. The chicken are kept alive after you received the first batch of antibodies for two weeks (up to day 77). Within this time you can decide whether you need additional antibodies or if you want to finish the project. A fifth immunization is performed to obtain the titer, when we have enough antigen available. Additional immunizations, egg collections and antibody preparations are possible over a long period of time.
From time to time chicken delay the laying of eggs over a small period of time. In this case the antibody preparation is delayed for a short period of time. We minimize this risk by selecting chicken that are healthy and currently lay eggs.
An Overview of our chicken egg yolk antibody services can be found on the purification section.

Immunization Schedule for Chicken Egg Yolk Antibodies
Day 63 Day Protocol
1Preimmune Egg Yolk IgG/IgY, approx. 90% pure
11. Immunization
142. Immunization
283. Immunization
354. Immunization
45-55Collection of the eggs
56Optional: 5. Immunization
63Preparation of Eggs
77Project Elongation or Termination
Production of antibodies from up to 200 egg yolks per year.

Chicken Antibody Purification

Ready to use Antibodies from chicken are included in all our service packages. PrepI antibodies are comparable with raw sera from mammals and are included in the packages A001/A002. PrepII or ProteinL purification (included in A003) leads to a pure whole IgY fraction. This fraction can be superior in many applications.
Antigen specific Affinity Purifications are available from the PrepI preparation. Just add the affinity purification to your order. For use in cell cultures we recommend the low endotoxin, high specific and sterile filtered antibodies (PrepIII). For further information or suggestions write us an email: info@davids-bio.com.

Purification Options for Chicken Egg Yolk Antibodies
PrepI PrepII PrepIII Affinity Purification
Cat.NoA001 (incl. 1 hen)
A002 (incl. 2 hens)
A012
A003 (incl. 1 hen)
R003
R006R017
Chicken Antibody ProductionAntibody Purification ColumnCell Culture FlasksAntibody Purification Columns
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ProcedurePreparationChromatographyAntigen bound to resinAntigen bound to resin
Comparable withRaw SerumProteinAPrepIIIAffinity Purification
ProductWhole IgY fraction in bufferHigh purity, whole IgY fractionSpecific Antibodies
Low Endotoxin
Sterile
Antigen Specific Antibodies
Purity70 - 90%90 - 98%
Amount (approx.)10 eggs corresponds to 100 ml Serum10 eggs corresponds to 100 ml Serum0.1 - 10 mg (10 eggs)0.5 - 10 mg (10 eggs)
Applications
Optimal forELISAImmuno-Histo-Chemistry (IHC)
in vivo Cell Culture Applications
Fluorescent Activated Cell Sorting (FACS)
WesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
PricePrice ListPrice ListPrice ListPrice List
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Additional Services

Davids always adapts to your special needs. Alternative immunization schedules and additional services are available. Collecion and Purification of large amount of eggs, isolation of lymphocytes from spleen, bone marrow and other organs as well as the RNA isolation are available.

Additional Services for Chicken Egg Yolk Antibodies
Cat.No. Description Amount Catalog Price
A012Additional Immunization, Housing for 30 days, Collection and PrepI10 eggsPrice List
R003Additional Immunization, Housing for 30 days, Collection and PrepII10 eggsPrice List
A705ELISA Raw Data incl. graphFrom 1 PreparationPrice List
Final Bleed (Antiserum)1 chickenPrice List
N101Spleen Cell Isolation1 chickenPrice List
N301Bone Marrow Cell Isolation1 chickenPrice List
T901RNA Isolation from Cells (1 x 10^8)1 organPrice List
P075.010anti-egg IgG matrix for immunoprecipitation1 mlPrice List

Advantage of Egg Yolk Antibodies

The table compares the advantages and disadvantages of Chicken Egg Yolk Antibodies from birds with Serum Antibodies from mammalians. IgG are identical to IgY as it is a filtrate of the serum. The antibodies can be used like sera from mammals.

Chicken Egg Yolk Advantages
Advantage Disadvantage Solution
No binding at ProteinA/ProteinGLess backgroundNo ProteinA or ProteinG purificationOur special purification methods (PrepI, PrepII) turn to equal or better antibodies
No activation of complement factorsLess background-
No binding to Fc of antibodiesLess background-
No binding to rheumatoid factor--
Lipids/Lipoproteins-Reduced activityPurification like PrepI, PrepII or PrepIII to remove the lipids and lipoproteins
Yield of IgGapprox. 50 - 100 mg/egg results in 1 - 2 g per month-

Order Process

The order process is very simple. Just send the antigen for immunization to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The immunizations usually start at the same day when the antigen arrives in our lab. Provide the following information to fasten up the start and help us to connect your antigen with your order

  1. Your Antigen
  2. Concentration & Buffer Conditions of your Antigen
  3. Our Offer, our order form or your official PO
  4. VAT number (only EU Customers, not German Customers)

If you want to order peptide antibodies just send us your protein sequence and we suggest you peptides that represent your protein. For more information: Peptide Antibodies