For the antibody production nearly any kind of antigen can be used. Usually proteins, peptides, SDS-Gel pieces, DNA, cells, virus particles or small molecules like toxins, haptens or drugs are used for the production of polyclonal or monoclonal antibodies.
Some of the antigens need a preparation before immunization, especially when the antigenicity is low. To improve the antigens for the immunizations a lot of methods like conjugation, liposome introduction or the usage of Davids Cell Preparation KIT is recommended.
Table #1 shows the recommended preparation of your antigens. Table #2 shows the recommended buffers for the antigens.
If you have any questions regarding your antigens, you can just send us an email: firstname.lastname@example.org.
Production of your antibodies with any kind of antigen. We start with the immunizations as soon as your customer provuded antigen arrives or as soon as we synthesized your antigen.
When you have purified protein available, the native protein can be used as antigen. For proteins that are difficult to purify, or when the purified protein shows multiple bands in a SDS-gel you can use the SDS-Gel Extraction service. Here you cut your specific protein band from the gel and we extract your protein from the SDS-gel pieces for the immunization.
For proteins you only know the sequence from, you can use our Peptide Antibody Service. Here we are looking for peptides that represent your protein. Our prediction program and our scientists predict the antigenicity, solubility and epitopes of the peptides. This service includes peptide prediction prior to order, synthesis and conjugation.
In addition we offer the immunization with antigens like DNA, Cells, Virus Particles, Toxins or Small Molecules. Please have a closer look at the handling with these antigens, as each antigen needs its individual preparation.
Please have a look at the table to see what kind of antigens are suitable for the immunizations of rabbits, chicken, mice, rats and guinea pigs. In addition some antigens need a preparation to optimize the antigenicity or the presentation to the immune system.
|Protein||We use your protein for the immunization||We start the immunizations immediately after your protein arrives|
|Protein Expression||We produce your antigen||Expression of your plasmid for the antibody production|
|Protein (SDS-Gel)||Cut the gel bands from your protein out of the gel||Save expensive and time consuming purification steps|
|Peptides||We can synthesize and conjugate your peptides or use your ones||Let us produce your peptide or send us your peptide for the conjugation and immunization|
|Cells||Use our cell immunization kit to prepare for immunizations||Send us your cells prepared with our cell immunization KIT|
|DNA||We prepare your DNA for the immunizations||Send us your DNA and we prepare it for direct immunization|
|Small Molecules||We prepare your small molecules for the immunization||Please contact us when you want to send small molecules|
You can choose any non-toxic buffer. Please have a look at the table of substance concentrations to have a guideline which buffers are fine for the immunizations. The maximum concentrations in the table are for antigens in a concentration of 1 mg/ml.
|Buffer Conditions||Maximum Concentration|
|Dithiothreitol (DTT)||2 mM|
|Citrate or Acetate||100 mM|
We perform ELISA titer determinations as quality control for our antibody services. With proteins extracted from SDS-gel or cells as antigen the ELISA results may differ to the real titer. Not every cell binds to ELISA plates without optimizing for the specific cell line. The same is for denaturated proteins covered with SDS. Please ask us if you have any additional questions.