Antigens for your Custom Antibody Production

For the antibody production nearly any kind of antigen can be used. Usually proteins, peptides, SDS-Gel pieces, DNA, cells, virus particles or small molecules like toxins, haptens or drugs are used for the production of polyclonal or monoclonal antibodies.

Preparation of Antigens

Some of the antigens need a preparation before immunization, especially when the antigenicity is low. To improve the antigens for the immunizations a lot of methods like conjugation, liposome introduction or the usage of Davids Cell Preparation KIT is recommended. Table #1 shows the recommended preparation of your antigens. Table #2 shows the recommended buffers for the antigens.
If you have any questions regarding your antigens, you can just send us an email: info@davids-bio.com.

Antigen Selection

Antigen
Selection

Antigen Details

Detailed Antigen Information

Buffer Requirements

Buffer Requirements

Additional Information

Additional Information


Antigen Selection - What Antigen is the best for my Application

Production of your antibodies with any kind of antigen. We start with the immunizations as soon as your customer provuded antigen arrives or as soon as we synthesized your antigen.

Protein Available

When you have purified protein available, the native protein can be used as antigen. For proteins that are difficult to purify, or when the purified protein shows multiple bands in a SDS-gel you can use the SDS-Gel Extraction service. Here you cut your specific protein band from the gel and we extract your protein from the SDS-gel pieces for the immunization.

Protein not available

For proteins you only know the sequence from, you can use our Peptide Antibody Service. Here we are looking for peptides that represent your protein. Our prediction program and our scientists predict the antigenicity, solubility and epitopes of the peptides. This service includes peptide prediction prior to order, synthesis and conjugation.

More Antigens for Antibody Generation

In addition we offer the immunization with antigens like DNA, Cells, Virus Particles, Toxins or Small Molecules. Please have a closer look at the handling with these antigens, as each antigen needs its individual preparation.

Antigen

Please have a look at the table to see what kind of antigens are suitable for the immunizations of rabbits, chicken, mice, rats and guinea pigs. In addition some antigens need a preparation to optimize the antigenicity or the presentation to the immune system.

Antigens for Antibody Production.
Antigen Instructions Information
ProteinProteinWe use your protein for the immunizationWe start the immunizations immediately after your protein arrives
Protein ExpressionProtein ExpressionWe produce your antigenExpression of your plasmid for the antibody production
SDS-PAGE GelProtein (SDS-Gel)Cut the gel bands from your protein out of the gelSave expensive and time consuming purification steps
PeptidesPeptidesWe can synthesize and conjugate your peptides or use your onesLet us produce your peptide or send us your peptide for the conjugation and immunization
CellsCellsUse our cell immunization kit to prepare for immunizationsSend us your cells prepared with our cell immunization KIT
DNADNAWe prepare your DNA for the immunizationsSend us your DNA and we prepare it for direct immunization
ConjugationSmall MoleculesWe prepare your small molecules for the immunizationPlease contact us when you want to send small molecules

Buffer Requirements

You can choose any non-toxic buffer. Please have a look at the table of substance concentrations to have a guideline which buffers are fine for the immunizations. The maximum concentrations in the table are for antigens in a concentration of 1 mg/ml.

The buffer can contain the following ingredients. Examples for buffer contents.
Buffer Conditions Maximum Concentration
Urea2 M
NaCl200 mM
Imidazole200 mM
Dithiothreitol (DTT)2 mM
Citrate or Acetate100 mM
Glycerol10%
SDS0.1%
Triton X-1000.2%
EDTA1 mM
TRIS100 mM

Additional Information

We perform ELISA titer determinations as quality control for our antibody services. With proteins extracted from SDS-gel or cells as antigen the ELISA results may differ to the real titer. Not every cell binds to ELISA plates without optimizing for the specific cell line. The same is for denaturated proteins covered with SDS. Please ask us if you have any additional questions.