Production and Development of Monoclonal and Polyclonal Antibodies

From your antigen to the final antibodies. Fast and easy solution by Davids. Just send your antigen with the order form or a copy of your offer, that you received by email (info@davids-bio.com) to Davids (Davids Biotechnologie GmbH, Roentgenstrasse 3, 93055 Regensburg, Germany). We start with the antibody production and you receive an immunization schedule.

Antigen
Preparation
Antigen
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Immunization
Immunization Schedule
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Purification
Antibody Purification
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Shipping
Antibody Shipping

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Davids produces high quality antibodies since more than 20 years. We offer a wide range of common species for your antibody production services. This includes the immunization of your antigen to produce polyclonal and monoclonal antibodies. You will find additional information for antigen recommendations on our antigen page.
Besides the production of your antibodies we take care of the purification and modification of your antibodies. With efficiency and high binding capacity your antibodies are purified from antisera, egg yolk fractions and cell culture supernatants from IgG and IgM producing clones.

Polyclonals

Polyclonal Antibodies

Monoclonals

Monoclonal Antibodies

Antibody Production

Antibody Production

Purification

Antibody Purification



Polyclonal Antibodies Production

Polyclonal antibodies are the antibodies of choice when you need cost efficient and fast produced high affinity antibodies. The polyclonal antibodies recognize multiple epitopes of the antigen. This usually leads to a strong signal with a broader background. The background can be reduced with our purification methods like affinity purification. With polyclonal peptide antibodies we are able to produce antibodies that target an epitope region which can be helpful when you want to distinguish between protein families or if you want to detect multiple families with one antibody batch.

Overview of our polyclonal antibody services. The amount represents the product from one animal.
Rabbit Chicken Mouse Rat Guinea Pig
Rabbit Antigen RecommendationsChicken Antigen RecommendationsMouse Antigen RecommendationsRat Antigen RecommendationsGuinea Pig Antigen Recommendations
Preimmune0.5 ml0.5 ml PrepI0.02 ml0.02 ml0.05 ml
Test Serum0.5 mlOptional: 0.5 ml0.02 ml0.02 ml0.02 ml
Final Product40 - 90 ml
Serum
10 eggs
Purified (PrepI)
0.2 - 0.5 ml
Serum
2 - 10 ml
Serum
5 - 15 ml
Serum
Available PurificationsPrepI
ProteinA
Affinity Purification
Depletion
PrepI included
PrepII
PrepIII
Affinity Purification
PrepI
ProteinA
Affinity Purification
Depletion
PrepI
ProteinA
Affinity Purification
Depletion
PrepI
ProteinA
Affinity Purification
Depletion
More InformationRabbit
Antisera
Chicken
Antibodies
Mice
Antisera
Rat
Antisera
Guinea pig
Antisera

Monoclonal Antibodies Development

When you need antibodies that recognize one single epitope and antibodies that are reproduced constantly, monoclonal antibodies are the perfect choice for you. In addition every batch of your produced antibodies has no or very low variability. Monoclonals are very specific and thus usually have less background in many applications. In combination with their reproducibility, monoclonals are very good for assays that need a high reproducibility.
All our monoclonal antibody development services includes that you receive all rights for the developed clones after payment. You will receive the clone frozen to produce and purify the antibodies in your lab. If wanted we store your clones in our lab for the additional productions, purifications or just as a backup.

Monoclonal Antibody Services.
Development Service Mouse Development Service Rat In vitro Monoclonal Antibodies
Monoclonal Mouse AntibodiesMonoclonal Rat AntibodiesIn vitro Monoclonal Antibodies
Animal Species4 x Mice3 x RatNo animals needed
Test Bleed4 x 0.02 ml
includes ELISA titer determination
3 x 0.05 ml
includes ELISA titer determination
No animals needed
Fusion1 spleen from best mouse1 spleen from best ratSpleen from Mouse or Rat
ScreeningWe screen for positive clones
Multiple antigens are possible
We screen for positive clones
Multiple antigens are possible
We screen for positive clones
Multiple antigens are possible
You receiveAliquot of purified antibodies from best clones
Best clones frozen
Aliquot of purified antibodies from best clones
Best clones frozen
Aliquot of purified antibodies from best clones
Best clones frozen

Antibody Production from Hybridoma

Our production services include the production of monoclonal antibodies from your clones. The production of your antibodies is from clones developed by Davids for you or from clones that you send us. In addition we produce your proteins or antibodies from your plasmid with our transient transfection in CHO cell for example. For additional information go to our Production Page.

Antibody Purification (Sera, Egg Yolk, Cell Culture Supernatants)

Our well established purification methods for polyclonal and monoclonal antibodies include: Affinity Purifications, Purification with ProteinA or ProteinG and Depletions for antibodies from tagged antigens. If you want to use your antibodies in in vivo applications we highly recommend our PrepIII purification for ultra pure, endotoxin free and sterile antibodies, ready to use in cell culture experiments.
Cell culture supernatants from your monoclonal IgG or IgM clones are purified with multiple chromatography steps. For additional information go to our Purification Page.