Monoclonal Antibody Production

Hybridoma Production and Purification Service

Production and purification of antibodies from hybridoma cells (antibody generating clones) and cell culture supernatants. Please use Davids Monoclonal Development Service or give us your hybridoma or cell culture supernatants for the production and purification.

Antibody Production from Hybridoma

Davids produces and purifies antibodies from your hybridoma cell lines that generate antibodies. You can either send your hybridoma (clone) to Davids for production or utilize one that Davids already created for you previously (Monoclonal Antibody Development).

MAb Production by Davids

Davids exclusively utilizes spinner and roller flasks in in-house built bio reactors for production. The final product is high purity antibody fractions with minimal endotoxin levels. We have a large output range spanning from 10 mg to 10000 mg. Simply request your customized deal for large-scale projects. Table 1 illustrates some examples of antibody production and purification services. In addition, we offer the production in serum-free medium as well as the generation and purification of IgM or other antibodies that do not attach to ProteinA material.

	Production (5 - 10 mg)	Production (30 mg)	Production (100 mg)
Cat.No.	M301-010	M301-030	M301-100
We need	Your clone frozen (-80°C)	Your clone frozen (-80°C)	Your clone frozen (-80°C)
Schedule
Growing	3 weeks	3 weeks	3 weeks
Production	8 weeks	10 weeks	13 weeks
Purification	1 weeks	1 weeks	2 weeks
Product
Purification	ProteinA included	ProteinA included	ProteinA included
Targeted Amount	5 - 10 mg	30 mg	100 mg
Quality Control	Determination of Protein Concentration	Determination of Protein Concentration	Determination of Protein Concentration
Mycoplasm Test	+	+	+
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Antibody Production Schedule

Please bear in mind that the schedule and yield are dependent on the clone generating the antibody. The time is calculated for clones with a yield of 10 mg/l. Please inquire about special pricing for bigger or smaller volume production. Furthermore, ProteinA does not operate with every clone. Please check our antibody purification services for these clones.

Is the Hybridoma really monoclonal (subclone)?

Verify that the clone has been subcloned. Clones that have not been subcloned may not be monoclonal and, as a result, lose production rate or activity over time. Please utilize our subcloning service if you are unsure whether the clone is genuinely monoclonal.

Purification of Supernatants

Our production services cover the purification with ProteinA or other materials of your supernatants from antibody generating clones. In special cases, further polishing processes are required to remove polymers or other undesirable compounds. We propose polishing steps after ProteinA as an answer for this.
We provide ProteinA independent purification for IgM subclasses or antibodies that do not bind to ProteinA. This approach is used to purify IgM or small antibody fragments, among other things.

	ProteinA	Independent Purification	Polishing
Cat.No.	M501	M502	M503
Schedule
Schedule	1 weeks	1 - 2 weeks	1 - 2 weeks
Product
Available for	IgG	IgG IgM Fragmented Antibodies	IgG IgM Fragmented Antibodies
Method	ProteinA	Ion-Exchange-Chromatography (IEX) Size-Exclusion-Chromatography (SEC)	Ion-Exchange-Chromatography (IEX) Size-Exclusion-Chromatography (SEC)
Amount	We are able to purify small and large amounts
Quality Control	Protein Concentration	Protein Concentration	Protein Concentration
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Transient Transfection

Transient transfection of proteins and antibodies in suspension cultures of CHO cell cultures provides the benefit of appropriately posttranslationally modified proteins. Protein yields range from 1 to 200 mg per liter of medium. You are welcome to send us your transient transfection plasmid and we generate your protein. You may also send us your plasmid in modest amounts (50 ng) and we will make your plasmid for transient transfection.

	Plasmid Amplification	Transient Transfection in CHO	General Transient Transfection
Cat.No.	E500	E501	E502
We need	50 ng of your transient transfection plasmid	1 - 5 mg Plasmid	1 - 5 mg Plasmid
Schedule	5 days	4 - 8 weeks	4 - 8 weeks
Purification	Production with reduced Endotoxin	ProteinA Other Purifications Available	ProteinA Other Purifications Available
Quality Control	Agarose Gel	SDS-PAGE & Protein Concentration	SDS-PAGE & Protein Concentration
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Additional Services

Cat.No.	Description	Amount	Catalog Price
M006	Subclass Determination: IgG/IgM	1 clone	Price List
M008	Storage of one cryoconserved clone: 3 vials, 1 year	1 clone, 1 year	Price List
-	Sterile Filtration	1 fraction
B001	ELISA Development: Antigen	1 clone
B002	ELISA Development: Primary Antibody	1 clone
-	WesternBlot Analysis	1 clone
-	Immuno-Histo-Chemistry Analysis	1 clone
-	Up-Concentration of IgG	1 fraction

Order Process & Clone Shipping

We keep the order process very simple. You can send us your clone or supernatant to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

We start with the production and purification as soon as your material arrives. Please add the following information to help us connect your order with the material:

1. Type of antibody
2. Species (mouse, rat, guineapig, ...), Subclass (IgG, IgM, ...)
3. Is the antibody subcloned
4. If known type of purification (ProteinA, ProteinG, Independent)
5. Our Offer, our order form or your official PO
6. VAT number (only EU customers, not required for German customers)

You have the choice to send us your clone or use one of your clones developed or stored at Davids. You can ship your clone according to the table:

Type
Frozen cells	Freeze the cells with 5 - 10% DMSO or 5% glycerin. You may add 10 - 20% serum.
Cell cultures	Centrifuge the cells in log-phase (max. 300 x g), discard the supernatant and resuspend the pellet in fresh medium. Close the lid and send it in the vial together with a non-frozen ice pack.