Antibody Purification from Antisera and other fractions

Purification of antibodies and proteins from antisera, egg yolk, supernatants and other antibody fractions. Purification of material from your lab or antisera that were produced by Davids with your antigen.

Antisera Purification

Antisera Purifications

Chicken Antibody Purification

Egg Yolk Purifications

Supernatant Purification

Supernatant Purification

Order Process

Order Process


Antibody Purification from Antisera

Purification of antisera is done for better results in your applications. Applications like WesternBlot, IHC, DotBlot or ELISA profit by purification of the antisera. The antibody fraction is for example high specific (antigen specific affinity purification) or high pure (Whole IgG ProteinA purification). The table shows our most ordered purification methods, though we purify larger amounts of antisera, supernatants and egg yolks as well. Just ask for your special offer. For our antibody production services including the immunizations go to our Rabbit or Small Rodent (Mice, Rats, Guinea pigs) section.

Antisera Purification
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (10 - 50 ml)
R007-2 (50 - 100 ml)
R008 (10 - 50 ml)R003 (10 - 50 ml)R101 (anti-tag)
R102 (anti-E. coli)
Antibody Purification ColumnsHigh Efficient Antibody Purification ColumnAntibodies in Cell CultureAntibody Depletion
Duration5 - 10 days5 days5 - 10 days5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
Additional InformationPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrixPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrixOptional: Send the fraction for purification
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
ImmunoHistoChemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
ImmunoHistoChemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
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Antibody Purification from Egg Yolk

The preparation of egg yolks to receive the antibodies is one of the most important steps to receive the best chicken antibodies. Davids Preparation I removes most lipids and lipoproteins and can be used directly in most applications. Preparation II removes remaining lipids and lipoproteins and make a polishing step by chromatography. Preparation III performs an affinity purification at a low endotoxin level and sterile filtration for direct use in cell culture. In addition the antigen specific affinity purifications for your egg yolk antibodies is recommended for most applications and can be performed after Preparation I. For our chicken egg yolk antibody services including the immunizations go to our Chicken Egg Yolk Antibody Section.

Purification Options for Chicken Egg Yolk Antibodies
PrepI PrepII PrepIII Affinity Purification
Cat.NoR001R003R006R017
Purification of Chicken AntibodiesHigh Purity Antibody PurificationCell CultureAntibody Chromatographic Purification Columns
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ProcedurePreparationChromatographyAntigen bound to resinAntigen bound to resin
Comparable withRaw SerumProteinAPrepIIIAffinity Purification
ProductRaw Antibodies in bufferHigh purity, raw antibodiesSpecific Antibodies
Low Endotoxin
Sterile
Specific Antibodies
Purity70 - 90%90 - 98%Antigen SpecificAntigen Specific
Amount (approx.)10 eggs corresponds to 100 ml Serum10 eggs corresponds to 100 ml Serum0.1 - 10 mg (10 eggs)0.5 - 10 mg (10 eggs)
Applications
Optimal forELISAImmuno-Histo-Chemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
WesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
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Purification of Supernatants from Monoclonal Antibodies

Davids offers the purification of your monoclonal antibody supernatants or the production & purification of your monoclonal antibody producing clone/hybridoma. Please have a look at our Monoclonal Antibody Production & Purification section. Alternatively you can leave the antibody development to us with our Monoclonal Antibody Development Service.

Order Process

We keep the order process very simple. You can send us your material to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The purification usually starts as soon as your material arrives. To fasten up the process and help us to connect your material with your order ensure you provide the following information:

  1. Your Antigen for affinity purifications only
  2. Your Antibody containing solution
  3. Your desired Purification
  4. Our Offer, our order form or your official PO
  5. VAT number (only EU Customers, not German Customers)