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Antibody Purification

Purification of antisera and antibody fractions

Antibodies and proteins are purified from antisera, egg yolk, supernatants, and other antibody fractions. Purification is available of material from your lab or antisera made by Davids using your antigen.

Antisera Purification

Antisera Purifications

Send us your antisera or let us perform with the immunizations and antiserum generation

Chicken Antibody Purification

Egg Yolk Purifications

Several egg yolk preparations are available for your specific needs, such as antigen specific affinity purifications.

Supernatant Purification

Supernatant Purification

Purification of your supernatants from hybridoma

Order Process

Order Process


Antibody Purification from Antisera

Purification of antisera is performed to improve the results of your applications. Purification of antisera benefits applications including WesternBlot, IHC, DotBlot, and ELISA. For example, the antibody fraction would be highly specific with "antigen specific affinity purifications" or highly pure with "whole IgG ProteinA purifications". Although we purify bigger volumes of antisera, supernatants, and egg yolks, the table represents our most ordered purification processes. Simply request your special deal. Visit our rabbit or rodent (mice, rats, guinea pigs) site for our antibody production services, which include vaccinations.


Antisera Purification
Affinity Purification ProteinA Cell Culture Purity Depletion
Cat.NoR007 (10 - 50 ml)
R007-2 (50 - 100 ml)
R008 (10 - 50 ml)R003 (10 - 50 ml)R101 (anti-tag)
R102 (anti-E. coli)
Antibody Purification ColumnsHigh Efficient Antibody Purification ColumnAntibodies in Cell CultureAntibody Depletion
Duration5 - 10 days5 days5 - 10 days5 days
Product Information
ColumnAntigen bound to resinProteinA bound to resinAntigen bound to resintag/E. coli bound to resin
Additional InformationPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrixPlease send your antigen (0.5 - 1 mg) for the creation of the affinity matrixOptional: Send the fraction for purification
ProductSpecific AntibodiesWhole IgG FractionSpecific Antibodies
Low Endotoxin
Sterile
Fraction without unwanted Antibodies
Applications
Optimal forWesternBlot
ImmunoHistoChemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
ImmunoHistoChemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
Depends on Depleted Fraction
PricePrice ListPrice ListPrice ListPrice List
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Antibody Purification from Egg Yolk

One of the most important steps in receiving the strongest chicken antibodies is preparing the egg yolks. Most lipids and lipoproteins are removed by Davids Preparation I, which may be utilized directly in most applications. Preparation II eliminates residual leftover lipids and lipoproteins and integrates a chromatographic polishing step. Preparation III involves a low endotoxin affinity purification and sterile filtering for direct use in cell culture. Furthermore, for most applications, antigen specific affinity purifications for your egg yolk antibodies are suggested and may be performed after Preparation I. Visit our Chicken Egg Yolk Antibody Section for more information on our services, including vaccinations.


Purification Options for Chicken Egg Yolk Antibodies
PrepI PrepII PrepIII Affinity Purification
Cat.NoR001R003R006R017
Purification of Chicken AntibodiesHigh Purity Antibody PurificationCell CultureAntibody Chromatographic Purification Columns
Duration5 days5 days5 - 10 days1 - 5 days
Product Information
ProcedurePreparationChromatographyAntigen bound to resinAntigen bound to resin
Comparable withRaw SerumProteinAPrepIIIAffinity Purification
ProductRaw Antibodies in bufferHigh purity, raw antibodiesSpecific Antibodies
Low Endotoxin
Sterile
Specific Antibodies
Purity70 - 90%90 - 98%Antigen SpecificAntigen Specific
Amount (approx.)10 eggs corresponds to 100 ml Serum10 eggs corresponds to 100 ml Serum0.1 - 10 mg (10 eggs)0.5 - 10 mg (10 eggs)
Applications
Optimal forELISAImmuno-Histo-Chemistry (IHC)
in vivo Applications
Fluorescent Activated Cell Sorting (FACS)
WesternBlot
Immuno-Histo-Chemistry (IHC)
Fluorescent Activated Cell Sorting (FACS)
ELISA
PricePrice ListPrice ListPrice ListPrice List
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Purification of Supernatants from Monoclonal Antibodies

Davids provides purification of your monoclonal antibody supernatants as well as the production and purification from your monoclonal antibody generating hybridoma. Please visit our Monoclonal Antibody Production & Purification section for further information. You can also outsource the antibody development to Davids using our Monoclonal Antibody Development Service.

Order Process

We keep the order process very simple. You can send us your material to:

Davids Biotechnologie GmbH
Roentgenstrasse 3
93055 Regensburg
Germany

The purification usually starts as soon as your material arrives. To fasten up the process and help us to connect your material with your order ensure you provide the following information:

  1. Your Antigen for affinity purifications only
  2. Your Antibody containing solution
  3. Your desired Purification
  4. Our Offer, our order form or your official PO
  5. VAT number (only EU customers, not required for German customers)